Abstract:

This study examines Brassica General Process UGT76G's role in dynamic stem epigenetic regulation and protection via isoleucine acid conjugation. Increasing crop resistance to drought, salt, and temperature changes relies only on this process. Poor accuracy in targeted plant path modification and inadequate gene editing methods limit agricultural stress resistance. Under varied environmental conditions, conventional breeding methods are delayed and unreliable. The recommended strategy uses CRISpen-Cas9 technology to correctly edit isoleucine conjugation pathway genes, focusing on UGT76G1, a gene involved in stem protection and stress tolerance. This approach increases epigenetic system control over stress-responsive genes through site-specific, efficient CRISpen-Cas9 gene editing. This allows direct management of crop environmental resistance mechanisms. Plant resilience may be targeted via agricultural breeding using CRISpen-Cas9 technology. Exact gene modifications improve stem epigenetic factor dynamic regulation and stress tolerance. Under harsh climatic conditions, this approach boosts crop growth, yield, and survival. As it creates abiotic-tolerant crops, the method is promising for future agriculture.